| Cities | Population |
| Fort Myers | 86,395 |
| Sanibel | 6,469 |
| Fort Myers Beach | 6,227 |
| Estero | 36,939 |
| Lehigh Acres | 135,272 |
| Alva | 2,596 |
| Bokeelia | 1,780 |
| Bonita Springs | 43,914 |
| Cape Coral | 194,016 |
| Buckingham | 4,036 |
| Burnt Store Marina | 1,793 |
| Captiva | 583 |
| Charleston Park | 218 |
| Cypress Lake | 11,846 |
| East Dunbar | 1,935 |
| Fort Myers Shores | 5,487 |
| Gateway | 8,401 |
| Harlem Heights | 1,975 |
| Iona | 11,756 |
| Lochmoor Waterway Estates | 4,204 |
| Matlacha | 677 |
| Matlacha Isles-Matlacha | 229 |
| McGregor | 7,406 |
| North Fort Myers | 39,330 |
| Olga | 1,952 |
| Page Park | 514 |
| Palmona Park | 1,353 |
| Pine Island Center | 1,854 |
| Pine Manor | 3,428 |
| Pineland | 407 |
| District 1 | Commissioner Kevin Ruane |
| District 2 | Commissioner Cecil Pendergrass, Chairman |
| District 3 | Commissioner Ray Sandelli, Vice chair |
| District 4 | Commissioner Brian Hamman |
| District 5 | Commissioner Mike Greenwell |
| District 4 | Debbie Jordan, Chair |
| District 1 | Mary Fischer, Vice chair |
| District 2 | Melisa W. Giovannelli |
| District 3 | Chris N. Patricca |
| District 5 | Gwynetta S. Gittens |
| District 6 | Betsy Vaughn |
| District 7 | Cathleen O'Daniel Morgan |
Lee County is represented in the United States House of Representatives by Byron Donalds of the 19th district and by Greg Steube of the 17th district. Unlike most urban counties, Lee County is a Republican stronghold in presidential elections. The last Democratic presidential candidate to win the county was Franklin D. Roosevelt in 1944.
